Antinuclear antibodies by indirect immunofluorescence and solid phase assays
نویسندگان
چکیده
منابع مشابه
Detection of antinuclear antibodies by solid-phase immunoassays and immunofluorescence analysis.
BACKGROUND Antinuclear antibodies (ANAs) are associated with several inflammatory rheumatic diseases. The aim of the present work was to evaluate enzyme immunoassays (EIAs) and compare them with classic immunofluorescent analysis (IFA) for the detection of ANA. METHODS Seven enzyme immunoassays were used in this study. All assays were applied as described by the manufacturers. Three populatio...
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BACKGROUND & OBJECTIVES Antinuclear antibodies (ANA) are serological hallmark of systemic lupus erythematosus (SLE). Conventionally, the test is carried out on human epithelial cells (HEp2) by indirect immunofluorescence (IIF) technique. Since culturing and maintaining HEp2 cells in the laboratory are labour intensive, in-house assays have given way to kits manufactured by commercial companies....
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BACKGROUND HEp-2 cells that overexpress the human 60-kDa SSA antigen have been used to improve sensitivity and specificity for the detection of anti-SSA antibodies by indirect immunofluorescence. We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA. METHODS The evaluation was done on 18 371 consecutive samples submitted to the labora...
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Two immunoglobulins secreted by hybridoma cell lines have been systematically investigated to determine if they could be used in solid-phase assays to give results comparable with those obtained by conventional liquid-phase radioimmunoassay (RIA). The antibodies, BQ.1 and BQ.2, bind with high specificity to the steroid hormone progesterone. The affinity constants, Ka, to 125I-labeled progestero...
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INTRODUCTION Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) ba...
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2019
ISSN: 0003-4967,1468-2060
DOI: 10.1136/annrheumdis-2019-215443